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Kit Instruction Manual This reagent is for research use only: This kit is used to determine the content of myoglobin (Mb) in horse serum, plasma and related liquid samples. Experimental principle: This kit uses the double antibody sandwich method to determine the level of horse myoglobin (Mb) in the specimen. The microplate was coated with purified horse myoglobin (Mb) antibody to prepare a solid phase antibody, and myoglobin (Mb) was sequentially added to the microcapsule of the coated monoclonal antibody, followed by HRP-labeled myoglobin (Mb). The antibody binds to form an antibody-antigen-enzyme-labeled antibody complex, which is thoroughly washed and then added to the substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The depth of the color is positively correlated with myoglobin (Mb) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of horse myoglobin (Mb) in the sample was calculated from a standard curve.
August 23, 2021