Silica gel relative to the ceramic tableware, plastic tableware, hardware, silica gel tableware harmony together with temperature, in which food is cold is hot, can protect the temperature of the food itself, reduce the loss of the change of temperature and a period of time in the silicone bowl or pot food can still maintain the original temperature, when use won't convey the temperature of the corresponding to the user. Moreover, the particularity of the silicone material itself, which is different from other materials, makes the products produced by it have an amazing effect. For example, landing silent food containers, after high temperature cooking, will not produce harmful substances. And tableware but fold, can knead, can turn over, chuai also does not take a space in the pocket, also won't adsorb smeary. Its own desiccant effect, will not because of long-term storage and moldy, qualitative change. Silicone Shovel,Silicone Kitchen Shovel,Silicone Leak Shovel,Silicone Cooking Shovel Ningbo Sunmoon Silicone Product CO.,Ltd , https://www.sunsilicone.com
And because of silica gel temperature resistant properties, heat resistance of 240 ℃ high temperature deformation, won't produce hardening phenomenon to 40 ℃, so the product is fully staffed, very practical, we don't have to classification of casual in a microwave, don't worry about the melting and aging and yellowing phenomenon, and it is easy washing, can use less or even do not use detergent containing chemical substances, practicability and convenience are very strong, very suitable for use in their life.
This reagent is for research use only: This kit is used to determine the content of Orexin A in human serum, plasma and related liquid samples. Experimental principle: This kit uses the double antibody sandwich method to determine the level of human Arexin A in the specimen. The microplate was coated with purified human Arexin A antibody to prepare a solid phase antibody, and the micropores of the coated monoclonal antibody were sequentially added with Orexin A and HRP-labeled Alixin A. The antibody (Orexin A) binds to form an antibody-antigen-enzyme-labeled antibody complex, which is thoroughly washed and then added to the substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The depth of the color is positively correlated with the Arexin A in the sample. The absorbance (OD value) was measured by a microplate reader at a wavelength of 450 nm, and the content of human Arexin A in the sample was calculated by a standard curve. Kit composition: Kit composition 48-well configuration 96-well configuration Storage instructions 1 part 1 part sealing film 2 pieces (48) 2 pieces (96) Sealed bag 1 1 enzyme label coated plate 1 × 48 1 × 96 2 -8 ° C preservation standard: 135pg / mL 0.5ml × 1 bottle 0.5ml × 1 bottle 2-8 ° C preservation standard dilution 1.5ml × 1 bottle 1.5ml × 1 bottle 2-8 ° C preservation enzyme standard reagent 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation sample dilution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation developer A solution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C preservation Reagent B liquid 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation solution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation concentrated washing solution (20ml × 20 times) × 1 bottle (20ml × 30 times) × 1 bottle 2-8 ° C preservation sample processing and requirements: 1. Serum: room temperature blood solidification for 10-20 minutes, centrifugation for about 20 minutes (2000-3000 rev / min). The supernatant is carefully collected, and if precipitation occurs during storage, it should be centrifuged again. 2. Plasma: EDTA or sodium citrate should be selected as an anticoagulant according to the requirements of the specimen. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm). The supernatant is carefully collected, and if a precipitate forms during storage, it should be centrifuged again. 3. Urine: Collect with a sterile tube and centrifuge for about 20 minutes (2000-3000 rpm). The supernatant is carefully collected, and if a precipitate forms during storage, it should be centrifuged again. The chest and ascites and cerebrospinal fluid are referred to. 4. Cell culture supernatant: When detecting secreted components, collect them in a sterile tube. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. When the components in the cells were detected, the cell suspension was diluted with PBS (pH 7.2-7.4), and the cell concentration reached about 1 million/ml. By repeated freezing and thawing, the cells are destroyed and the intracellular components are released. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again. 5. Tissue specimen: After cutting the specimen, weigh the weight. Add a certain amount of PBS, pH 7.4. It was quickly frozen and stored in liquid nitrogen for use. The specimen still maintains a temperature of 2-8 ° C after melting. A certain amount of PBS (pH 7.4) was added, and the specimen was homogenized by hand or homogenizer. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. One part of the package is to be tested, and the rest is frozen for use. 6. The specimens should be extracted as soon as possible after collection, and the extraction should be carried out according to the relevant literature. The experiment should be carried out as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20 °C, but repeated freezing and thawing should be avoided. 7. Samples containing NaN3 cannot be detected because NaN3 inhibits horseradish peroxidase (HRP) activity.
June 17, 2021